Work packages

WP1. UCNPs Synthesis, structural characterization, functionalization and biosafety tests.

Objectives:

Synthesis of FLUCNPs, FNDs, and GQDs suitable for the imaging of the biological tissues and cells.

Task 1.1. Synthesis of the FLUCNPs (INRS, UEF, ULM, UW, FTMC, UniSa, Platformina), M1-M36.

Task 1.2. Synthesis of the FNDs (ULM, UEF, UW, FTMC, UniSa, Platformina, INRS, Adamas), M1-M36.

Task 1.3. Fabrication of rare-earth ions doped GQDs (UEF, FTMC, ULM, Platformina), M1-M36.

Task 1.4. Structural characterization (FTMC, UEF, ULM, UW, FTMC, UniSa, Platformina), M1-M39.

Task 1.5. Functionalization (Platformina, FTMC, INRS, Adamas), M4-M40. Task 1.6. Biosafety (FTMC, UEF, Adamas, INRS), M4-M42.

Deliverables:

D1.1. Protocol and technology of UCNPs synthesis. (UEF, M18).

D1.2. Protocol and technology of optimized synthesis of UCNPs selected for FLORIN super-resolving imaging scheme. (UEF, M42).

WP2. Spectroscopic characterization of UCNP. Experimental validation of UCNP biosensing properties.

Objectives:

UCNPs spectroscopic characterization for further optimization of their synthesis and functionalization routes, biosensing tests, demonstration of Quantum thermometry.

Task 2.1. Spectroscopic characterization (FTMC, UEF, ULM, UW, INRS, Adamas), M1-M40.

Task 2.2. Biosensing. Nanothermometry (ULM, UEF, FTMC, UW, Platformina), M3-M44.

Task 2.3. Comparative analysis of upconverting /emission performance of UCNPs (FTMC, INRS, ULM, UW, Atomicus, UniSa), M6-M44.

Deliverables:

D2.1. Spectroscopic characterization of first series of UCNPs. (ULM, M18).

D2.2. Report on biosensing and quantum nanothermometry. (ULM, M44).

WP3. Multiphysics modeling of UCNP emission and sensing properties.

Objectives:

Theory of the bioimaging based on FLUCNPs, FNDs, and GQDs with a focus on stochastic emission regime; modelling of the neuron cell electrophysiological response on the UCNPs emission.

Task 3.1. Stochastic emission regime modelling (Atomicus, UniSa, INRS, UW, UEF), M1-M24.

Task 3.2. Theoretical description of imaging and object inference method (ULM, UW, Atomicus, UEF, UniSa, Adamas), M6-M30.

Task 3.3. Finite element method modelling of a neuron under external forces. (UniSa, FTMC, UEF), M1-M30.

Deliverables:

D3.1. Data sheet of the most important parameters and KPIs based on theory and verified neuron model (ULM, M30).

WP4. SOFISM / Q-ISM with UCNPs and AI based data and image processing.

Objectives:

Implementation of the SOFISM / Q-ISM imaging schemes with produced UCNPs and biological samples.

Task 4.1. Development of SOFISM and QISM techniques with biological samples (UW, UEF, INRS, Adamas, FTMC, Platformina), M6-M24.

Task 4.2. AI based data processing (Atomicus, ULM, UEF, UniSa), M12-M30.

Deliverables:

D4.1. FLORIN device prototype (UW, M24).

D4.2. AI based data processing software ready for practical bioimaging (ATOMICUS, M30).

WP5. Practical bioimaging. Optogenetics. Proof of concept experiments.

Objectives:

Demonstration of the super-resolution bioimaging and biosensing using fabricated nanoparticles.

Task 5.1. Preparation and imaging of neuron cultures (UEF, UW, UniSa, Platformina, Adamas, IRNS), M12-M36.

Task 5.2. Imaging and Optogenetics (UW, UEF, FTMC, Platformina, ULM, Adamas, INRS), M12-M48.

Deliverables:

D5.1. SOFISM/Q-ISM approach tested with AD affected cell samples (UW, M24).

D5.2. Optogenetics testing and validation of neuronal- and glia-specific outcome measures related to nanoparticles in in vitro disease models of AD. (UEF, M48).

WP6. Training, networking, dissemination, communication.

Objectives:

WP6 focuses on the training, broad dissemination and communication of FLORIN project results and networking activities that will be carried out continuously. The main objectives are: (1) effective and sustainable dissemination of knowledge among and beyond the members of the consortium; (2) coordination of knowledge management and other innovation-related activities; (3) coordination of workshops and training activities within the project; (4) coordination of communication and outreach activities to public; (5) promotion of international and inter-sectoral long-lasting collaborations.

Task 6.1. Plan of Exploitation and Dissemination of project results, M1-M48, UEF, all partners.

Task 6.2. Website, M1-M48, UEF with participation of all partners on the day-by-day basis.

Task 6.3. Organization of workshops, tutorials, trainings and consortium meetings, M1-M48, UEF, all partners.

Task 6.4. Dissemination of results, M1-M48, UEF, all partners. Task 6.5. Communication strategy and Outreach plan, M1-M48, UEF, all partners.

Deliverables:

D6.1. PEDR (UEF, M3)

D6.2. Project Web-site (UEF, M3); D6.3. Report on dissemination, communication activities and outreach (UEF, M24, M48).

WP7. Management. Data Management. Exploitation plan.

Objectives:

The overall objective of WP7 comprises planning, organization, coordination and controlling of the project implementation in order to accomplish the targeted goals. WP7 includes management of the secondments and the resources, risk and data management.

Task 7.1. Project coordination and management, M1-M48, UEF, all partners

Task 7.2. Coordination of secondments, M1-M48, UEF, all partners

Task 7.3. Risk management, M1-M48, UEF, all partners

Task 7.4. Data management, M1-M48, UEF, all partners

Deliverables:

D7.1. Data Management plan (UEF, M1);

D7.2. Progress reports (UEF, M12, M36); D7.3. Mid-term and final reports (UEF, M24, M48).