Targeted, quantitative metabolite measurements

Targeted, quantitative metabolite measurements

The LC-MS Metabolomics Center has developed methods for targeted quantitative analyses of various compounds. The methods available at the center (in co-operation with School of Pharmacy) are based on long-term experience in the analysis of specific compounds or compound groups with wide range of instrumentation (LC-MS/MS, GC-MS, HPLC-UV/DAD, HPLC-ECD). The development and validation of these methods follows the international quality guidelines.

As an example, quantitative analyses are available for the following compounds:

  • cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC)
  • dopamine and its metabolites
  • endocannabinoids
  • lysophosphocholines
  • noradrenaline and its metabolites
  • polyamines: free (DAP, PUT, CAD, SPD, SPM) monoacetylated (AcPUT, AcCAD, N(1)AcSPD, N(8)AcSPD, N(1)AcSPM) and diacetylated (DiAcPUT, DiAcCAD, DiAcSPD, DiAcSPM)
  • serotonin (5-HT)
  • steroids in serum and tissue: 11-deoxycortisol, 17-OH-pregnenolone, 21-OH-progesterone, aldosterone, androstanedione, androstenediol, androstenedione, androsterone, corticosterone, cortisol, cortisone, DHEA, DHT, estradiol, estrone, etiocholanone, pregnenolone, progesterone, testosterone
  • total fatty acid content
  • Targeted protein quantitation (GLUT1, OATP, LAT1, COX2, MRP, etc)
  • Pharmaceuticals (e.g., atorvastatin, alprenolol, atenolol, metoprolol, pindolol, bupivacaine, entacapone, tolcapone, levodopa, carbidopa, midazolam, nimodipine, NSAIDs, perphenazine, tamsulosine, buprenorphine)