Equipment

Equipment list

Opera Phenix Plus with on-board pipettor

Sensitive and fast spinning disc confocal microscope system
Five Solid State Excitation Lasers: 375 nm, 425 nm, 488 nm, 561 nm, 640 nm
Two large format sCMOS cameras (6.5 µm pixel size, up to 100 frames per second)
Flexible Filter Set with Narrow band filters (435-515 nm, 465-530 nm, 500-530 nm, 515-550 nm, 571-596 nm, 605-630 nm, 650-680 nm, 690-720 nm)
Three automated water-immersion obejctives (20x NA 1.0, WD 1.7 mm, 40x NA 1.1, WD 0.62 mm, 63x NA 1.15, WD 0.6 mm)
Two air objectives (5x Air objective NA 0.16, WD 12.1 mm, and 20x Air, NA 0.4, WD 8.28 mm)
Brightfield and Digital Phase Contrast imaging
Liquid Handling Kit with On-board Pipettor for automated pipetting of up to two reagents to 96- and 384-well plates
Environmental Control, Temp., Humidity & CO2

For recommended plate types, please contact Janne Capra (janne.capra@uef.fi)

INCUCYTE S3

High-throughput imaging station for live-cell and time-lapse experiments
Six plates for simultaneous automated acquisition and analysis of living cells
Incubator with temperature and CO2 control
CMOS camera with 4 x, 10 x and 20 x objectives
Image acquisition using visible light, green and red fluorescence
Modules for Standard imaging, Image Lock, Scratch Wound, Whole Cell, Dilution Cloning, Spheroid, Chemotaxis, Cell-by-cell and Neurotracker

ZEISS LSM800 Airyscan

Superresolution microscope for imaging of live and fixed samples with high resolution and sensitivity.
Inverted microscope with 10 x, 20 x, 40 x oil and 63 x oil –objectives
Incubator with temperature and CO2 control
Four lasers available: 405, 488, 555 and 640 nm.
Airyscan imaging mode for increased resolution and signal-to-noise ratio

ZEISS LSM700

Basic confocal microscope for both live and fixed cell and tissue samples.
Inverted microscope with 10 x, 20 x, 25 x water/oil, 40 x oil and 63 x oil –objectives
Incubator with temperature and CO2 control
Four lasers available: 405, 488, 555 and 640 nm

LEICA THUNDER IMAGER 3D TISSUE Slide scanner

Slide scanner for imaging of both bright-field and fluorescent samples
8-slide holder and six objectives (1.25x, 4x, 10x, 20x, 40x dry objectives and 63x oil immersion objective)
Fluorescence filters for blue (DAPI), green (FITC), yellow (rhodamin), near red (Texas Red) and far red (Y5)
Z-stack and time-lapse imaging possible
Advanced image stitching and computational image clearing capabilites

ISS M612 TCSPC/FLIM confocal microscope

Time-correlated single photon counting microscope capable of all fluorescence fluctuations spectroscopy measurements (FCS, FCCS, PCH, FLCS), including Scanning FCS, Raster Image Correlation Spectroscopy (RICS) and Number & Brightness (N&B), FRET and Fluorescence Lifetime Imaging (FLIM & PLIM) together with phasor analysis.

Picosecond supercontinuum laser (430 – 2000nm) with the tunable repetition rates from
0.01MHz to 200MHz
Two Hybrid PMTS
Mechanically hyperstable Galvano-controlled mirrors
Digital TCSPC data acquisition with a deadtime of 6 ns
60x N.A 1.2 water Immersion objective, 20x N.A. 0.75 dry objective, 10x N.A. 0.45 dry objective

NOVOCYTE QUANTEON Flow cytometer

4-laser and 25-detector flow cytometric analyzer enabling single-cell multi-parameter assessment of fluorescently stained cell samples
Automated start up and shutdown
Possibility run 96-well plates and 5 mL flow cytometry tubes
Easy-to-use analysis program

For more information on Novocyte, please contact Tuure Kinnunen (tuure.kinnunen@uef.fi)

BD FACSAria III Cell sorter

3-laser, 11 parameter cell sorter enabling cell sorting of fluorescently stained cell samples
Possibility to sort different sized cells/particles, and possibility to sort into tubes or 96-well plate.

For more information on BD FACSAria III, please contact Tuure Kinnunen (tuure.kinnunen@uef.fi)

IMAGE ANALYSIS WORKSTATION

With Imaris 10 For Core Facilities, ZEN, LAS X, Incucyte 2022B Rev2.

SOFTWARE DOWNLOADS

ZEN Lite is available for download from:
https://www.zeiss.com/microscopy/en/products/software/zeiss-zen-lite.html#getzenlite
Fill the form and you will get the link for installation via email.

LAS X for Leica Thunder Imager 3D Tissue can be downloaded from:
https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/downloads/

RESERVATIONS

OpenIris reservation system:

Reservation of Opera Phenix Plus and ISS M612 systems is done on OpenIris reservation system:
openiris.io

To request access to OpeIris, please contact Janne Capra (janne.capra@uef.fi)

University Outlook web access calendar:

ZEISS LSM700:

e-kuo-bio-zeiss_lsm700

ZEISS LSM800 Airyscan:

e-kuo-bio-airyscan

INCUCYTE S3:

e-kuo-bio-incucyteplate

Each plate (1-6) has a separate calendar.
When booking a plate, check other plates as well to make sure your experiment fits!

Leica THUNDER Imager 3D Tissue slide scanner:

e-kuo-bio-leica_slidescanner

Image analysis workstation:

room-kuo-bio-siRNA

Novocyte Quanteon flow cytometer:

e-kuo-kli-Novocyte-Quanteon-Flow-Cytometer-4044