Equipment

Location: Snellmania 3153

Sensitive and fast spinning disc confocal microscope system with fluorescence, brightfield and digital Phase Contrast imaging. Liquid Handling Kit with On-board Pipettor for automated pipetting of up to two reagents to 96- and 384-well plates.
Environmental Control, Temp., Humidity & CO2.

Harmony software for data analysis. Harmony is also available on a separate workstation, and the computer is communicating with the Opera Phenix Plus computer to have the data available wirelessly and automatically.

Instrument specifics

• Available laser
  • 375 nm, 425 nm, 488 nm, 561 nm, 640 nm
• Available objectives
  • Air objectives
    • 5x Air objective NA 0.16, WD 12.1 mm
    • 20x Air, NA 0.4, WD 8.28 mm
  • Automated water-immersion objectives
    • 20x NA 1.0, WD 1.7 mm
    • 40x NA 1.1, WD 0.62 mm
    • 63x NA 1.15, WD 0.6 mm
• Narrow band filter Sets
  • 435-515 nm, 465-530 nm, 500-530 nm, 515-550 nm, 571-596 nm, 605-630 nm, 650-680 nm, 690-720 nm
• Cameras
  • Two large format sCMOS cameras (6.5 µm pixel size, up to 100 frames per second).

For recommended plate types, please contact Janne Capra ([email protected]).

Location: Snellmania 3156

Two high-content imaging stations for live-cell and time-lapse experiments. Six plates for simultaneous automated acquisition and analysis of living cells per instrument. Incubator with temperature and CO2 control.

Instrument specifics

• Available fluorescent lines
  • S3: green (ex:440-480, em: 504-544) and green and red (ex: 565-605, em: 625-705)
  • SX5: green, orange and NIR
• Available Objectives
  • Air objectives
    • 10x Air
    • 20x Air
• Cameras
  • CMOS camera

Modules for Standard imaging, Image Lock, Scratch Wound, Whole Cell, Dilution Cloning, Spheroid, Chemotaxis, Cell-by-cell, Advanced Label-free Classification (S3 only), Neurotracker and AI Cell Health (SX5 only).

Location: Snellmania, 3151

Super-resolution microscope for imaging of live and fixed samples with high resolution and sensitivity. Airyscan imaging mode for increased resolution and signal-to-noise ratio. Incubator with temperature and CO2 control

Instrument specifics

• Available laser
  • 405, 488, 555 and 640 nm
• Available objectives
  • Air objectives
    • 10x
    • 20x
  • Oil-immersion objectives
    • 40x NA 1.1, WD 0.62 mm
    • 63x NA 1.15, WD 0.6 mm
• Filter Sets
• Cameras

Location: Snellmania 3151

Basic confocal microscope for both live and fixed cell and tissue samples.
Incubator with temperature and CO2 control

Instrument specifics

• Available laser
  • 405, 488, 555 and 640 nm
• Available objectives
  • Air objectives
    • 10x
    • 20x
  • Oil-immersion objectives
    • 40x NA 1.1, WD 0.62 mm
    • 63x NA 1.15, WD 0.6 mm
  • Water/oil-immersion objectives
    • 25x
• Filter Sets
• Cameras

LSM 700 user manual

Location: Snellmania 3151

8-Slide scanner for imaging of both bright-field and fluorescent samples. Z-stack and time-lapse imaging possible. Advanced image stitching and computational image clearing capabilities.

Instrument specifics

• Available fluorescence lines
  • white light source
• Available objectives
  • Air objectives
    • 1.25x
    • 4x
    • 10x
    • 20x
    • 40x
  • Oil-immersion objectives
    • 63x NA 1.15, WD 0.6 mm
• Filter Sets
  • blue (DAPI), green (FITC), yellow (rhodamine), near red (Texas Red) and far red (Y5)
• Cameras

Location: Snellmania 3155

Fully motorized wide-field fluorescence microscope that can image using both fluorescent and brightfield, is able to take Z-stacks and tile images, and has deconvolution algorithms for image quality improvement.

Booking: Evident calendar

Instrument specifics

• Available fluorescence lines
  • white light source
• Available objectives
  • Air objectives
    • 4x, NA 0.13
    • 10x, NA 0.3
    • 20x, NA 0.8
    • 40x, NA 0.95
  • Oil-immersion objectives
    • 60x, NA 1.42
    • 100x, NA 1.45
• Filter Sets
  • FITC, rhodamine, TRITC, Cy5 and Cy7
• Cameras

Location: Snellmania 3151

Time-correlated single photon counting microscope capable of all fluorescence fluctuations spectroscopy measurements (FCS, FCCS, PCH, FLCS), including Scanning FCS, Raster Image Correlation Spectroscopy (RICS) and Number & Brightness (N&B), FRET and Fluorescence Lifetime Imaging (FLIM & PLIM) together with phasor analysis. Mechanically hyperstable Galvano-controlled mirrors
Digital TCSPC data acquisition with a deadtime of 6 ns

Instrument specifics

• Available fluorescence lines
  • Picosecond supercontinuum laser (430 – 2000nm) with the tunable repetition rates from 0.01MHz to 200MHz
• Available objectives
  • Air objectives
    • 10x, NA 0.45
    • 20x, NA 0.75
  • Water-immersion objectives
    • 60x, NA 1.2
• Filter Sets
  • GFP, rhodamine
• Cameras
  • Two Hybrid PMTS

With Imaris 10 For Core Facilities, ZEN, LAS X, and Incucyte.

With Harmony (for Opera Phenix Plus data analysis).

Two MultiFlo FX units, one equipped with two syringe and two peristaltic pumps for automated dispensing and aspiration of up to four reagents, and the second equipped with AMX media exchanger module that allows gentle dispensing and aspiration of liquid from low-attachment cell, organoids and spheroids.