EPHYS CORE FACILITY

IN VITRO AND EX VIVO ELECTROPHYSIOLOGY CORE FACILITY

     

Electrophysiology provides a unique way of investigating cell and tissue functionality. This is important for high quality science not only in basic cell biology and disease investigation but also in the continuously growing fields of stem cells, tissue engineering and therapy development.

Our in vitro / ex vivo electrophysiology core facility has instrumentation for measurement of single cell electrical and functional properties, including combined fluorescence imaging and patch clamp / intracellular recording systems for cell cultures and tissue slices as well as multielectrode array platform for measurement of neuronal circuits. We provide services on Ca-imaging on cells and slices, patch-clamp experiments, intracellular voltage recordings, cellular and subcellular high speed confocal imaging of ions or voltage, extracellular voltage recordings (cell cultures, small tissues) and MEA measurements of network activities.

We offer personal training, help and guidance for experimental planning, tissue or cell processing and data analysis. We also offer a full service package including experimental design, conduction of the measurements and the following analyses, and provide education in electrophysiology. You can check for more information here.

Sample type and experiment type are sometimes very research question specific and thus need special protocols to be followed and optimized.

Instrument usage:

Ca-imaging unit: 26€/hour

Total hourly rate for university internal projects (from 16.00 to 08:00), cheaper evening and weekend pricing reduces the workload of day hours by 15€/hour.

Sales price to outsiders (incl. VAT 24%, and 24% overhead, 20% margin) 44€/hour Consultation price for outsiders  (incl. VAT 24%, and 24% overhead, 20% margin, indirect employee costs 45%) 62€/hour.

Multichannel systems MEA: 10€ /hour

Sales price to outsiders 10€/hour (incl. VAT 24%, and 24% overhead, 20% margin) 19€/hour.

Consultation price for outsiders (incl. VAT 24%, and 24% overhead, 20% margin, indirect employee costs 45%) 62€/ hour.